Cellomet

  • Biomolecular interactions
  • Proteomics
  • Systems Biology
« bonnes pratiques de laboratoires » procedure requested by national centers as INSERM or CNRS
Localization
Place Amélie Raba-Léon
DEMANDE D'UTILISATION

Contact

Description

CELLOMET is a transfer technology platform linked to the MRGM academic laboratory and offering research services in the field of cell metabolism, with a particular emphasis on energy metabolism. Typically CELLOMET will work for academic laboratories to decipher the peculiarities of cancer cells energy metabolism and for pharmaceutical companies to decipher the mode of action of anti-cancer drugs with specific action on energy metabolism.
The strength of CELLOMET, in addition to specific tools for metabolic evaluation, is our recognized expertise in the field of energy metabolism and our capacity to solve related questions both in the clinical or the drug discovery settings

Activity

  • in vitro or ex vivo metabolic and bioenergetic assays organised in work packages to suit your drug discovery and/or clinical development needs (please enquire for further information on different packages):
  • Packages 1-3: classic measurements of energy metabolism.
  • Package 4: Comprehensive global mapping of metabolic networks: Shotgun quantitative proteomic and metabolic network rewiring analysis based on label-free proteomics and carbon isotope metabolic tracing; Predictive analysis of metabolic outcome (bioinformatic analysis); Predictive metabolic biomarker analysis and compound mechanism of action.
  • Package 5: Tissue Metabolism Investigation; Respiration of living tissue samples; Shotgun quantitative proteomic and metabolic network rewiring analysis based on label-free proteomics and carbon isotope metabolic tracing; High throughput tissue analysis of metabolic targets
  • Package 6: Metabolic biomarkers and signature; High identification and validation of targeted biomarkers and surrogate markers/biomarkers identification by targeted mass-spectrometry; Analysis of omic data for metabolic signature discovery

Infrastructure and equipment

Proprietary metabo-proteomic evaluation process for the deciphering of large-scale changes in metabolic network topology bank of cancer cell models and a biobank of human tumor and corresponding non-cancer tissues Bioinformatic tools to study the functional impact of those modifications of cell metabolism Bioenergetics investigations: Oroboros, Seahorse XF96, SAFAS Xenius... Metabo-proteomic analyses: LTQ-Orbitrap XL, Sequest, Mascott Zeiss Vivatome microscope to study the mitochondrial network in 4D Western Blot or Oxyblot using a Chemidoc (Biorad) and an Odyssey (Licor) Mass-spectrometry analyses (subcontracted to the proteomic facility of Bordeaux University): Ultimate 3000 nanoLC system (Dionex) coupled to a nanospray LTQ-Orbitrap XL mass spectrometer (ThermoFinnigan, San Jose, CA)

Publication

  1. Emerging concepts in bioenergetics and cancer research: metabolic flexibility, coupling, symbiosis, switch, oxidative tumors, metabolic remodeling, signaling and bioenergetic therapy.
  2. Obre E, Rossignol R. Int J Biochem Cell Biol. 2015 Feb;59:167-81 Redox Homeostasis and Mitochondrial Dynamics. Willems PH, Rossignol R, Dieteren CE, Murphy MP, Koopman WJ. Cell Metab. 2015 Aug 4;22(2):207-18.
  3. The presence of wild type p53 in hematological cancers improves the efficacy of combinational therapy targeting metabolism. Allende-Vega N, Krzywinska E, Orecchioni S, Lopez-Royuela N, Reggiani F, Talarico G, Rossi JF, Rossignol R, Hicheri Y, Cartron G, Bertolini F, Villalba M. Oncotarget. 2015 Jul 30.
  4. Anaplerotic metabolism of alloreactive T cells provides a metabolic approach to treat graft-versus-host disease. Glick GD, Rossignol R, Lyssiotis CA, Wahl D, Lesch C, Sanchez B, Liu X, Hao LY, Taylor C, Hurd A, Ferrara JL, Tkachev V, Byersdorfer CA, Boros L, Opipari AW. J Pharmacol Exp Ther. 2014 Nov;351(2):298-307.

Collaboration

  • Pharmaceutical and biotech industries
  • Cosmetic industry
  • Food industry
  • Academics (grant programs and international networks)
  • University
  • Canceropole GSO
  • Toxicology laboratories