A 3D Spheroid Model for Glioblastoma

Journal of visualized experiments, Apr 2020

Guyon J, Andrique L, Pujol N, Røsland GV, Recher G, Bikfalvi A, Daubon T.

doi: 10.3791/60998.



Two-dimensional (2D) cell cultures do not mimic in vivo tumor growth satisfactorily. Therefore, three-dimensional (3D) culture spheroid models were developed. These models may be particularly important in the field of neuro-oncology. Indeed, brain tumors have the tendency to invade the healthy brain environment. We describe herein an ideal 3D glioblastoma spheroid-based assay that we developed to study tumor invasion. We provide all technical details and analytical tools to successfully perform this assay.